Chemical localizome analysis using fission yeast for understanding nuclear function

Spliceostatin A(SSA)是一种有效的抗肿瘤微生物代谢物FR901464的甲基化衍生物，被发现在哺乳动物细胞中引起前mRNA的积累和翻译。SSA抑制剪接和与剪接复合体结合的前-mRNA的核保留。在这项研究中，我使用分裂酵母进行了化学定位分析，以确定其亚细胞定位在SSA处理后发生变化的蛋白质。我发现有相当数量的蛋白质改变了它们在核仁上的定位，这些蛋白质参与了核糖体的生物发生，表明SSA影响核糖体的加工。

Spliceostatin A (SSA), a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. SSA inhibits splicing and nuclear retention of pre-mRNA binding to the splicing complex. In this study, I performed chemical localizome analysis using fission yeast for identifying proteins whose subcellular localizations were changed upon treatment with SSA. I found that significant number of proteins altered their localization to the nucleolus and that those proteins were involved in ribosomal biogenesis, suggesting that SSA impinges on ribosomal processing.