Cooperative formation of ruthenium-platinum dinuclear complex and its application to gene detection

越来越多的人认识到，新的单核苷酸多态性（SNP）基因分型的化学和平台可以提供简单操作的高质量信号，这对高通量基因分析是非常有益的。我们报道了一项研究的分子设计的DNA探针携带钌络合物，最初具有一个特征的相对同位素丰度。这里用于检测目标DNA的分子方法依赖于基于蛋白质剪接反应的DNA模板化钌复合物释放的原理。每个探针都含有寡肽片段，这是该反应的基本元素。由于两个探针的序列被设计成与靶标形成三元串联双工，它们的辅助单元相互面对，为钌配合物的释放提供了微环境。因此，可以预期具有钌特征图案的成分的质量在反应前后会发生变化。另一方面，含有一个碱基缺失匹配的目标DNA不能作为有效的模板。最后，我们成功研制了钌或铂配合物的客观探针。

There has been growing recognition that the chemistries and platforms for the new SNP (Single Nucleotide Polymorphism) genotyping that provide quality signals with simple operation are quite beneficial for high-throughput gene analyses.We report a study on the molecular design for the DNA probe carrying a ruthenium complex which has originally a characteristic relative isotopic abundance. The molecular approach used here for the detection of the target DNA relies on the principle of the DNA-templated release of the ruthenium complexes based on the protein splicing reaction. Each probe has the oligopeptide moiety which are essential elements for this reaction. Since the sequences of the two probes are designed so as to form a ternary tandem duplex with the target, where their auxiliary units face each other to provide a microenvironment for the release of the ruthenium complex. Therefore, it is expected that the mass of the ingredient with a characteristic pattern of ruthenium would change before and after a reaction. On the other hand, the target DNA containing one base miss match could not serve as an effective template. Finally, we succeeded the objective probes carrying ruthenium or platinum complexes.