Fundamental study for the cultural development of anaerobic oxidation of methane with denitrification.

在淡水生境中，甲烷厌氧氧化与反硝化的意义在很大程度上是未知的。首先，我们尝试在厌氧条件下丰富利用甲烷和硝酸盐进行反硝化的微生物群落。接种部分河流沉积物和厌氧产甲烷颗粒污泥进行培养。厌氧颗粒污泥具有较高的反硝化活性。然而，反硝化速率仍然太低，无法研究哪些参数影响甲烷反硝化活性。因此，我们开发了提高液体培养基中甲烷等气体浓度的生物反应器。开发的生物反应器能够在水相中分布微气泡，并保持高浓度的氧气和甲烷等气体。

The significance of anaerobic oxidation of methane with denitrification in fresh water habitats is largely unknown. First, we tried to enrich the microbial community that denitrify with methane and nitrate under anaerobic condition. A portion of a river sediment and anaerobic methanogenic granular sludge were inoculated for the culture. High denitrifying activity was observed in the anaerobic granular sludge. However, the denitrification rate was still too low to investigate what parameters influence the methane denitrifying activity. Therefore, we developed the bioreactor which increased gas concentration such as methane in the liquid culture medium. The developed bioreactor was able to distribute microbubbles in the water phase and to keep high concentration of gas such as oxygen and methane.