Novel insights into the effect protecting DNA from radiation-induced damage : identification of relevant factors involved in paracrine epithelial-mesenchymal cell interactions

正常上皮细胞和间充质细胞通过分泌可溶性因子维持旁分泌串扰。观察到的这些细胞间相互作用的结果之一是，在用异源条件培养液相互处理后，这两种类型的细胞中辐射诱导的DNA损伤程度都得到了调节(减少)。分泌到培养液中的可溶性因子可能是旁分泌因子保守网络的一部分，该网络以背景模式工作，不需要细胞的任何特殊预处理来诱导。本研究试图以甲状腺原代上皮细胞和正常人成纤维细胞产生的细胞因子为模型，鉴定这些可溶性因子，并阐明其减轻遗传毒性损伤的机制。

Normal epithelial and mesenchymal cells maintain paracrine cross-talk through secreted soluble factors. One of the observed outcomes of these intercellular interactions is the modulation(decrease) of the extent of radiation-induced DNA damage in both types of cells after reciprocal treatment with heterologous conditioned medium. Soluble factors secreted into medium are presumably a part of the conserved network of paracrine factors operating in a background mode that does not require any special pretreatment of the cells for induction. This study attempted to indentify such soluble factors particularly focusing on cytokines produced by primary thyroid epithelial cells and normal human fibroblasts as a model, and elucidation of the mechanism by which genotoxic insult is diminished.