Effect of combined exposures to multiple chemicals and ultraviolet rays on recent increase of skin cancer-from a view point of histone modifications

由于组蛋白的修饰在基因表达和肿瘤发生中起着重要作用，已成为许多领域的研究热点。在这项研究中，我们研究了几种化学物质对组蛋白修饰的影响。侧流烟、甲醛等改变了部分组蛋白的修饰（组蛋白H3的磷酸化（S10）、组蛋白H3的乙酰化（K9、K14）和组蛋白H2 AX的磷酸化（S139））。组蛋白H3（S10）的磷酸化与原癌基因c-fos和c-jun的诱导有关，在组蛋白修饰显著的情况下，UVB诱导的细胞死亡增强。这些结果表明，几种化学物质对组蛋白的修饰可能会改变对DNA损伤剂的敏感性，表明组蛋白修饰对皮肤癌的增加有贡献。

Histone proteins have become a focus of research in many fields because their modifications have an important role in gene expression and cancer. In this study, we examined the influence of several chemicals on histone modifications. Side-stream smoke, formaldehyde, etc. changed the modifications of some histones(phosphorylation of histone H3(S10), acetylation of histone H3(K9, K14) and phosphorylation of histone H2AX(S139)). Phosphorylation of histone H3(S10) related with induction of proto-oncogenes, c-fos and c-jun. In the conditions of remarkable histone modifications, UVB-induced cell death was enhanced. These results suggested that histone modifications by several chemicals might change the sensitivity to DNA damaging agents, indicating the contribution of histone modifications to increase of skin cancer.