Large DNA molecule introduction into animal, plant and yeast cells using Type IV secretion system

一种从IncP型质粒衍生的IV型分泌系统（T4SS）作为一种新的基因导入真核细胞的方法被应用。我们发现酿酒酵母线粒体呼吸突变体的转化效率有所提高。利用线粒体功能抑制剂，成功建立了一种简单高效的大肠杆菌酿酒葡萄球菌基因导入方法。此外，我们首次观察到应用该T4SS将基因从大肠杆菌直接转移到高等植物细胞。

One of Type IV secretion systems (T4SS), which is derived from an IncP type plasmid, was applied as a novel gene introduction method into eukaryotic cells. We found that mitochondrial respiratory mutants in S. cerevisiae showed enhanced transformation efficiency. By using inhibitors of mitochondrial function, we successfully established a simple and efficient gene introduction method into S. cerevisiae from E. coli. In addition, we firstly observed direct gene transfer from E.coli to higher plant cells by applying this T4SS.