Verification of prolonged toxicity of tributyltin chloride toward marine bacteria in sediments of busy ports

三丁基氯化锡（TBTCl）在沉积物中通过吸附矿物而存在。从日本忙碌的盆栽中分离出10种耐tbt的海洋细菌。从东京湾检疫锚地分离到的光杆菌sp. TKY1对TBTCl的耐受性最强。即使在10^<12的情况下，分离物也能存活。单个细胞中有8b>个TBT^+分子。在100μM TBTCl浓度下，观察该分离物对tbt敏感弧菌的存活细胞数的变化。与没有分离物的情况下相比，观察到弧菌的存活细胞数量减少了两个数量级，这表明耐TBT的海洋细菌可能通过在细胞表面积累TBT^+来破坏海洋微生物的多样性。

Tributyltin chloride(TBTCl) has still been existed in sediments by adsorption to minerals. We isolated 10 species of TBT-tolerant marine bacteria from busy pots of Japan. Photobacterium sp. TKY1, which was isolated from quarantine anchorage in Tokyo Bay, showed the most tolerant ability toward TBTCl. The isolate survived even when the number of 10^<12. 8> molecules of TBT^+ was associated with a single cell. Changes in the number of surviving cells of TBT-sensitive Vibrio sp. was examined in the presence of the isolate when 100μM of TBTCl was existed. Two order reduction of the number of surviving cells of Vibrio sp. was observed in comparison with that in the absence of the isolate, indicating that the TBT-tolerant marine bacteria might have contributed to the disruption of marine microbial diversity through accumulation of TBT^+ onto cell surface.