Studies on molecular mechanisms underlying the insect olfactory associative learning

为了了解CS-US结合的分子基础，我首先研究了Kenyon细胞中表达的受体和离子通道，然后研究了传递US和CS信号的神经递质对它们的调节。本研究主要研究了M受体激活对Na~(++)激活的K~(++)通道和电压激活的Ca~(2+)~(2+)通道的调制及其可能的下游途径，涉及NOS/NO/cGMP/PKG信号转导途径。结果表明，M_1受体激活可通过激活PLC、Ca/CAM复合体和NOS/NO/cGMP/PKG信号通路，降低双斑蟋蟀(Gryllus Bimaculatus)蘑菇体Kenyon细胞K~(2+)、Na~(2+)和Gt~(2+)通道的开放概率(NPO)，增加Ca~(2+)通道的NPO。

To understand the molecular basis of CS-US association, first I investigated the receptors and ionic channels expressed in Kenyon cells and second their regulation by neurotransmitter substances which convey US and CS signals. The present study mainly focused on the modulation of Na^+-activated K^+and voltage-activated Ca^<2+> channels induced by muscarinic receptor activation and its possible downstream pathway involving NOS/NO/cGMP/PKG signaling. The present results indicate that muscarinic type of M_1 receptor activation decreases the open probability(NPo) of K_<Na> channels and increases NPo of Ca^<2+> channels via the sequential activation of PLC, Ca/CAM complex and NOS/NO/cGMP/PKG signaling cascades in Kenyon cells isolated from the mushroom body of the cricket Gryllus bimaculatus.