Study of rhodopsin-ion pumps by electrical measurement focusing on proteorhodopsin

在本研究中，我们研究了细菌视紫红质吸附在一个薄的聚合物膜上产生的光电流。对不同光强下实验结果的分析表明，细菌视紫红质产生的电动势与光强无关。光电流的pH依赖性表明，细菌视紫红质在pH 6左右能产生最大电动势。我们构建了一个类似细菌视紫红质的蛋白视紫红质突变体，发现其质子吸收和释放的顺序发生了变化。我们还建立了离子泵的一般模型。

In the present study, we examined the photoelectric current generated by bacteriorhodopsin adsorbed on a thin polymer film. An analysis of the experimental results obtained at various light intensities suggested that the electromotive force generated by bacteriorhodopsin was independent of the light intensity. The pH dependency of the photoelectric current suggested that the bacteriorhodopsin could generate the maximum electromotive force at around pH 6.We constructed a mutant of proteorhodopsin which mimics bacteriorhodopsin and found a change in the order of proton uptake and release. We also constructed a general model for ion pumps.