Molecular mechanisms of circadian regulation in CAM

为了从分子水平上阐明CAM的昼夜节律调控机制，本研究从中胚草、羽叶荠、克氏原球茎等CAM植物中克隆了CAM相关基因ppcD、Ppck、Ppc1、McPpck 1、Mod1的5'侧翼序列。fedtschenkoi。瞬时表达分析表明，在这些植物的基因组中存在阿坝、乙烯、干旱和盐胁迫以及昼夜节律的顺式调控元件，并存在Myb和Dof的结合位点。堆叠μ m足以驱动报告基因的表达。我们采用农杆菌介导的植物体内转化方法，将其转化到M.堆积这个。子叶、侧芽和种子的转化率分别为20%、90%和70%。

To elucidate molecular the mechanisms for circadian regulation of CAM, the 5' flanking region of CAM-related genes(ppcD, Ppck, Ppc1, McPpck1, Mod1) were isolated from CAM plants such as Mesembryanthemum crystallinum, Kalanchoe pinnata, K. fedtschenkoi. In the regions of these plants, cis-regulatory elements responsible for ABA, ethylene, drought and salt stresses and circadian regulate and the binding sites of Myb and Dof were found. Transient-expression assays indicated that the sequence 1600 to-1100 of McPpck of M. crystallinum was sufficient to drive the expression of the reporter gene. We applied Agrobacterium-mediated in planta transformation procedure to M. crystallinum. Transformation rates using cotyledons, side shoots and seeds were 20%, 90% and 70%, respectively.