Engineering of oxygen-requiring properties in Corynebacterium glutamicum

我们鉴定了与谷氨酸棒杆菌的微需氧生长相关的六个基因：Cgl0807、Cgl1102、Cgl0600、Cgl1427、Cgl2857和Cgl2859。其中，编码胞苷酸激酶的Cgl1427基因被证明在细胞在低氧浓度下生长时在DNA合成中起重要作用，这表明DNA合成可能成为氧限制下生长的限制因素。同时，从降低需氧量本身的角度出发，设计了一个C。谷氨酸菌株的糖酵解途径，可以产生NADPH代替NADH，并显示其作为生物技术过程的宿主的潜力。

We identified six genes relevant to the microaerobic growth of Corynebacterium glutamicum ; Cgl0807, Cgl1102, Cgl0600, Cgl1427, Cgl2857, and Cgl2859. Among these, the Cgl1427 gene encoding cytidylate kinase was shown to play an important role in DNA synthesis when cells were grown under low oxygen concentrations, suggesting that DNA synthesis might become a limiting factor for growth under oxygen limitation. Meanwhile, from the viewpoint of reducing oxygen requirement itself, we engineered a C. glutamicum strain with a modified glycolytic pathway that can generate NADPH instead of NADH, and showed its potential as a host for biotechnological processes.