Physiological analysis of potential of fructan-accumulating transgenic rice for biomass material

我们研究了孕穗期水稻在低温下蔗糖代谢和运输相关基因表达水平的变化。在12℃时，水稻原穗中蔗糖含量下降。基因表达分析表明，OsSUT和OsSUS、OsINV的表达水平可能与低温条件下水稻植株蔗糖代谢和分配的变化有关。库区组织中葡萄糖和果糖含量较高。果聚糖累积大米的含量也高于非转基因大米。这些结果可能是由于转基因水稻在诱导果聚糖合成或降解过程中释放单糖所致。

We investigated changes in the expression levels of genes related to sucrose metabolism and transport in tissues of booting-stage rice exposed to a chilling temperature. Decrease in sucrose content in primitive panicles of rice was observed at 12℃. The gene expression analysis suggested that expression levels of OsSUT and OsSUS, OsINV might be related to changes in sucrose metabolism and partitioning in rice plants under a cold condition. Contents of glucose and fructose were higher in sink tissues. The contents were also higher in fructan-acumulated rice than in non-transgenic rice. These results might be due to the release of monosaccharides during induced fructan synthesis or degradation in transgenic rice.