Analyses of Translocation Intermediates Formed during Protein Import into Chloroplasts in Higher Plants

核编码的叶绿体蛋白质作为前体翻译后，利用胞质中嵌入双被膜的独特的易位子进入叶绿体。在有限的能量条件下，前体被捕获在易位子上，形成早期易位中间体。在研究过程中，我建立了分析前体与易位子组分之间的蛋白质-蛋白质相互作用以及易位子内相互作用的实验系统。

Nuclear-encoded chloroplastic proteins are imported into chloroplasts by utilizing the unique translocon embedded in the double-envelope membranes from the cytosol after translated as precursors. Under limited energy conditions, precursors are trapped at the translocon to form the early-translocation intermediates. During the research period, I have established the experimental systems to analyze the protein-protein interactions between precursors and the translocon component(s) and intra-interactions within the translocon.