Development of methods for identification and quantitation of fentanyl related designer drugs and their metabolites

为了证明滥用毒品，必须检测和鉴定从滥用毒品者那里获得的尿液中的代谢物，这些代谢物反映了毒品的特殊化学结构。尽管α-甲基芬太尼是芬太尼及其相关化合物的代表性设计药物，但该麻醉剂的主要代谢物并不反映该药物特殊的化学结构。在这项研究中，我们能够检测和鉴定四种次要代谢物，它们反映了口服麻醉剂的大鼠尿液中α-甲基芬太尼的特殊化学结构。此外，我们不仅可以建立从尿液或血液中固相微萃取α-甲基芬太尼的方法，还可以建立尿液或血液中药物的气相色谱/质谱联用定量方法。

In order to prove narcotic abuse, detection and identification of its metabolites, which reflect peculiar chemical structure of the narcotic, in urine obtained from an abuser of the drug is essential. Though α-methylfentanyl is the representative designer drug of the fentanyl and its related compounds, major metabolites of the narcotic do not reflect peculiar chemical structure of the drug. In this study, we were able to detect and identify four minor metabolites, which reflect peculiar chemical structure of the α-methylfentanyl in urine obtained from rats to which we gave the narcotic orally. In addition, we could develop not only the method for solid-phase microextraction of the α-methylfentanyl from urine or blood but also that for quantitation of the drug in urine or blood with gas chromatography(GC)/mass spectrometry(MS).