精神機能に及ぼす甲状腺ホルモン作用におけるRCAN/カルシニューリン系の役割

RCAN（regulators of calcineurin）2由甲状腺素（T3）诱导，并控制钙调磷酸酶（CaN）的活性。CaN是一种在中枢神经系统中大量存在的蛋白磷酸酶，据报道，它通过敲除和过表达在学习记忆或精神状态中出现异常。由于甲状腺功能减退导致脑RCAN 2的表达减少，因此RCAN 2深入参与脑功能，我们推测甲状腺素对脑功能的影响是通过调节RCAN 2的基因表达来证明的。本研究通过对先前建立的RCAN 2基因工程小鼠（敲除小鼠）的形态学和行为学分析来研究RCAN 2蛋白在脑功能中的作用。RCAN 2 KO小鼠正常出生，未观察到与野生型相比的异常。以C57 BL/6 J为同源系，进行回交，使回交世代的10 ~ 100倍， ...更多信息 11只（N10或11）F2小鼠，并进行行为学分析。此外，我们将小鼠存放在理化学生物资源中心。在新生、生长期和成年中，与野生型小鼠相比，形态学变异在脑中不具有RCAN 2KO小鼠，并且需要未来更详细的研究。作为测量RCAN 2 KO小鼠的行为量的结果，差异[优效性/KO小鼠、野生型小鼠和异型小鼠的自发行为量]不可接受。然而，KO小鼠的行为量在移动到新情况（用于测量的新笼子）后立即减少。此外，我认为这种差异与其他行为试验（高架十字迷宫试验、Y字迷宫试验、强迫游泳试验、恐惧症条件学习试验）测量的情绪行为和学习能力无关。由理化学生物资源中心实施的“日本小鼠诊所”正在进行分析。少

RCAN (regulators of calcineurin) 2 is induced with a thyroxin (T3) and it controls the activity of a calcineurin (CaN). CaN is a protein phosphatase which exists in a CNS at a rich, and it is reported that unusuallies arise in a learning and a memory, or a mentation by the knock out and overexpression. Since the expression of cerebral RCAN2 reduced due to the hypothyroidism, RCAN2 participated in the cerebral function deeply and we assumed that the effect to the cerebral function of a thyroxin was demonstrated through the regulation of gene expression of RCAN2. This study examined the role in the cerebral function of RCAN2 protein through the morphological and behavioral analyses of the previously established RCAN2 genetically engineered mouse (knock out mouse). A RCAN2 KO mice is born normally and the abnormality compared with a wild type was not observed. We established as a congenic strain to C57BL/6J line, which carried out the back, cross, and made the backcross generation's 10 to … More 11 (N10or11) F2 mice, and provided the behavioral analyze. Moreover, we deposited the mice to the Riken bioresource center. In a nascent, a growth period, and an adult, a morphological variation does not have a RCAN2KO mice in a brain compared with a wild type mice, and a future still more detailed investigation is called for. As a result of measuring the amount of behaviourals of a RCAN2 KO mice, the difference [superiority / the amount of spontaneous behaviourals of KO mice, a wild type mice, and a hetero type mice] was not accepted. However, the amount of behavioral of KO mice was decreasing immediately after moving to a novel situation (novel cage for a measurement). Furthermore, I considered the difference to be an out-of thing to the emotional behavior and learning ability that have been measured by other behavioral trials (an elevated type crux maze trial, Y character maze trial, a forced-swimming trial, phobia conditional learning tests). The analyze by the "Japan mouse clinic" which a Riken bioresource center carry out is under perform. Less