Exploration of pathogenetic mechanism for hair diseases through dermal fibroblasts

广崎无毛大鼠（hrr - df）真皮成纤维细胞原代培养经多次传代后形成多核大成纤维细胞。这些细胞的增殖能力较低。包括犊牛血清、tgf - β和bFGF在内的几种生长刺激均未能促进hrr - df的细胞增殖。HHR背侧皮肤人工皮肤缺损，创面愈合迟缓。这些结果表明hrr - df对生长刺激的敏感性受损。

Primary culture of the dermal fibroblasts derived from Hirosaki hairless rat (HHR-DF) develops multinuclear large fibroblasts after multiple culture passages. These cells have low proliferation ability. Several growth stimuli including calf serum, TGF-beta, and bFGF failed to promote cell proliferation of HHR-DF. Artificial skin defect on the dorsal skin of HHR showed retarded wound healing. These results suggest that HHR-DF has an impaired sensitivity to growth stimuli.