Analysis on factors relating metastasis in human gastric cancer cell line focusing on the metastasis patterns

为了阐明肿瘤转移的机制，我们在裸鼠体内建立了高度淋巴转移、肝转移和腹膜播散的模型，然后对这些细胞中与转移相关的几个因素进行了表征。我们利用cDNA微阵列技术研究了肿瘤转移相关基因的生物学特性和mRNA水平。并对肿瘤转移抑制物质的利用情况及肿瘤转移潜能与血管生成因子的关系进行了研究。目前认为，胃癌细胞系中内皮素的表达水平可能与肝转移有关，而VEGF-C可能与淋巴结转移有关。骨桥蛋白也可能参与胰腺癌细胞的肝转移。

To elucidate the mechanisms of cancer metastasis, we established highly lymphatic, liver metastatic and peritoneal disseminated models in nude mice, and then characterized several factors related to metastasis in these cells. We investigated several biological properties and mRNA levels of differentially-expressed genes involved in cancer metastasis by cDNA macroarray. We also conducted the availability of metastasis inhibition substances and the relation between metastatic potential and angiogenesis factorts. To date, Endothelin expression level in gastric cancer cell line might contribute to the liver metastasis and VEGF-C to the lymphatic metastasis. Osteopontin might also contribute to the liver metastasis in pancreatic cancer cell line.