Application of endoscopic cell sheet transplantation for colorectal

在人口腔黏膜上皮细胞片预防ESD后狭窄的临床研究的基础上，我在小型猪身上进行了应用细胞片治疗结直肠ESD所致溃疡的基础实验。我在一只小型猪身上进行了结直肠ESD，然后用成纤维细胞片植入。然而，猪的分离细胞生长不稳定。因此，我决定改用口腔粘膜细胞片。我们的研究结果表明，结直肠细胞片移植是一种良好的短期治疗方法。穿孔可以用细胞板修复，从而降低并发症的风险。然而，这种可能性还需要进一步研究。

Based on clinical research of regenerative medical treatments using human oral mucosal epithelial cell sheets to prevent constriction after esophageal ESD, I conducted basic experiments on miniature pigs whereby cell sheets were applied to ulcerations caused by colorectal ESD. I conducted colorectal ESD on a miniature pig, followed by engraftment using fibroblast cell sheets. However, the isolated cells of the pig grew unstably. Therefore I decided to use oral mucosal cell sheets instead. The results of our study show that colorectal cell sheet transplantation is a good short-term treatment. It is possible that perforations can be repaired with cell sheets, decreasing the risk of complications. However, this possibility requires further study.