Morphological and functional analyses of osteoclast-like cells using time-lapse imaging by video-enhanced microscopy

通过肌动蛋白染色的破骨细胞样细胞建立稳定的荧光条件，分析空泡结构的形态学变化，观察破骨细胞在骨吸收过程中胞内结构的形态学变化及酸分泌机制。随着骨吸收活性的增强，可见细胞内液泡结构发生了形态变化，并向较大的细胞偏心分布。加入骨吸收剂艾卡顿素后，破骨细胞样细胞的活性发生变化。这种分布似乎是增强骨吸收活性的破骨细胞样细胞的特征性变化。

We established the stable fluorescent condition by actin stained osteoclast-like cells and analyzed morphological changes of vacuole structures to visualize the morphological changes of intracellular structure and acid secretion mechanism in bone resorption process by osteoclast. As the bone resorption activity was growing, it was visualized that the intracellular vacuole structures morphologically changed and were eccentrically distributed to the bigger cells. After adding elcatonin, bone resorption agent, this activity of osteoclast-like cells changed. This distribution seems to be characteristic changes in osteoclast-like cells growing the bone resorption activity.