From analysis of osteopontin function to development of preventive and medical treatment for osteoporosis.

我们建立了表达骨桥蛋白(OPN)的转基因小鼠(OPN-TG)，OPN是一种主要由成骨细胞分泌的磷酸糖蛋白。我们发现OPN-TG小鼠与正常小鼠相比，血清中钙离子和I型胶原交联型N-四肽(NTX)含量增加。OPN-TG小鼠QCT显示骨密度降低，是一种有用的人类骨质疏松模型。此外，我们还需要对含有OPN相关蛋白或多肽的骨髓细胞进行体外分析。

We generated transgenic(OPN-TG) mice expressing osteopontin(OPN) which is a phosphoglycoprotein secreted by osteoblasts mainly. We found that an increase of calcium and type I collagen cross linked N teropeptide(NTx) in serum of OPN-TG mice, compared with normal mice. OPN-TG mice showed a decrease of bone density obtained by QCT and so is a useful model of human osteoporosis. Furthermore, we need in vitro analysis of the bone marrow cells with OPN-related proteins or peptides.