Pathogenesis of endometriosis by the inflammatory cytokines in three-dimension culture system

1. 我们检测了经血对这些免疫细胞产生细胞因子能力的影响。我们检测CD14+单核细胞和DC分别在含有2%或10%经血的培养基中培养24小时，用ELISA法测定上清液中白细胞介素-6和白细胞介素-8的浓度。我们证明经血可以诱导免疫细胞的细胞因子水平。这些结果提示月经逆行可能与子宫内膜异位症的发病机制有关我们考察了三维文化体系。从正常子宫内膜和巧克力囊肿内膜中获得子宫内膜和子宫内膜异位症间质细胞。我们使用Celltight X TM（SUMILON）。与单层培养系统相比，我们评估了TNFa对这些呼叫的影响。ELISA法检测IL-6和IL-8蛋白的表达。子宫内膜异位症基质细胞产生炎性细胞因子而不产生TNFa。与单层培养系统相比，TNFa的加入促进了炎症细胞因子的产生。这些结果提示炎症因子在子宫内膜异位症细胞的增殖中起自分泌因子和旁分泌因子的作用

1. We examined the effect of menstrual blood on the ability of cytokine production in these immune cells. We examined the CD14+monocyte and DC were cultured the containing 2% or 10% menstrual blood in the culture medium for 24 hours, and measured the concentration of interleukin-6 and interleukin-8 in the supernatants with ELISA. We demonstrated that the menstrual blood induced the cytokine levels in the immune cells. These results suggest that retrograde menstruation may be contributed to the pathogenesis of endometriosis.2 We examined the three-dimensional culture system. Endometrial and endometriotic stromal cells were obtained from normal endometrium and from chocolate cyst linings. We used Celltight X TM(SUMILON). We evaluate the effect of TNFa on those calls compared with monolayer culture systems. IL-6 and IL-8 protein production was examined by ELISA. Endometriotic stromal cells product the inflammatory cytokines without TNFa. The addition of TNFa promoted the production of inflammatory cytokines, compared with monolayer culture systems. These results suggested that the inflammatory cytokines work as the autocrine and paracrine factor for the proliferation of endometriotic cells