Basic research about the development of the allogeneic reconstituted skin equivalent which induces local immunotolerance

本研究以近交系大鼠为材料，从大鼠皮肤中分离角质形成细胞和成纤维细胞，并将角质形成细胞永生化（AK-E7），建立诱导局部免疫耐受的同种异体重组皮肤替代物。使用AK-E7与成纤维细胞的重建皮肤等同物显示出几乎正常的组织学表皮结构。转染IL-10 cDNA的成纤维细胞培养上清（AFB-IL 10）可抑制混合淋巴细胞反应。当使用AK-E7和AFB-IL 10的重建皮肤等效物移植到自体移植模型中时，其部分吸收在组织学上被识别。

Basic research was performed with inbred rat strain to develope the allogeneic reconstituted skin equivalent inducing local immunotolerance.Keratinocyte and fibroblast was derived from rat skin, and the keratinocyte was immortalized(AK-E7). The reconstituted skin equivalent, using AK-E7 with fibroblast, showed nearly normal histologic epidermal structure. The cultured supernatant of the fibroblast transfected with IL-10 cDNA(AFB-IL10) inhibited mixed lymphocyte reaction. As the reconstituted skin equivalent using AK-E7 and AFB-IL10 was grafted into autograft model, its partial take was recognized histologically.