Analysis for genetic alteration of oral precancerous lesions

本研究成功地建立了舌癌野生型癌变动物模型。该模型提供了宏观和微观结果的良好兼容性，以及对癌前病变的时间和部位特定变化的分析。在该模型中，癌前病变表现为弥漫性上皮不典型增生，随着致癌物给药时间的延长，组织学异型性增加，虽然停药降低了异型性，但进一步加重了病变的异型性，如局灶性隆起病变和浸润性癌。癌前病变中存在与双链DNA损伤修复相关基因的高水平表达，提示与浸润性癌不同。

In this study, we successfully established field-cancerization animal model of tongue carcinoma. The model provides well compatibility of macroscopic and microscopic findings and analysis for chronological and site specific changes of the precancerous lesion. In this model, the precancerous lesion shows diffuse epithelial dysplasia and the grade of histological atypia was increased during the carcinogen administration period, although interruption of the administration of the carcinogen brought decrease the atypia but further advanced lesions such as focal protrude lesion and invasive carcinoma.There is high level expression of genes related with double strands DNA damage repair in the precancerous lesions, and it suggests that different properties from in the invasive carcinoma.