Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/Phosphophoryn/Collagen complex

目的是建立新的组织再生治疗广泛再生的破坏牙周组织伴随垂直或水平骨缺损，由于严重的牙周炎，本研究的目的是评估骨髓间充质干细胞培养在FGF的动力学。胶原复合物作为体外再生支架。骨髓间充质干细胞在胶原凝胶中生长良好。FGF促进细胞生长的效果在培养的早期阶段表现出来，当在10 ng/ml的FGF浓度下培养14天时测定时，ALP活性已经过了峰值。培养14 d时，磷酸化蛋白的存在对ALP活性的影响呈剂量依赖性。未观察到钙含量的显著差异。本研究揭示了形成钙化硬组织形成基础的最佳生物材料的发展，表明应用3-D胶原凝胶细胞培养系统构建控制再生组织量的支架的可行性。

With the goal to establish new tissue regenerative therapy for extensive regeneration of destroyed periodontal tissues accompanying vertical or horizontal bone defects due to severe periodontitis, The aim of this study was to evaluate the kinetics of bone marrow-derived mesenchymal stem cells cultured in a FGF. collagen complex as a regenerative scaffold in vitro. Bone marrow-derived mesenchymal stem cells showed good growth in the collagen gel culture. The effect of FGF in promoting cell growth is exhibited at the early stage of culture, and the ALP activity has already passed the peak when assayed at 14 days of culture at FGF concentrations of 10 ng/ml. The ALP activity showed in the presence of phosphophoryn in a dose-dependent manner at 14 days of culture. A marked difference in calcium content was not observed. The present study sheds light on the development of an optimal biomaterial that forms the basis of calcified hard tissue formation, suggesting the feasibility of applying the 3-D collagen gel cell culture system for the construction of a scaffold that controls the amount of regenerating tissue.