Analysis of propagation manners of paramyxoviruses in neural cells.

麻疹病毒和尼帕病毒均可引起人类严重的急性或迟发性脑炎。在这项研究中，我们的目的是研究麻疹和尼帕病毒在神经细胞之间传播的机制。我们建立了原代神经细胞的AMCA（on-chip agarose microchamber array）系统，并阐明了MV向邻近细胞的传播方式。利用荧光标记的病毒蛋白，鉴定了一个新的结构域，该结构域是NiV N蛋白和P蛋白相互作用的重要结构域。此外，我们研究了NiV的非洲绿色猴通过使用EGFP表达的重组NiV的传播。结果表明，中枢神经系统的致死性感染发生在全身感染之后。

Both Measles and Nipah virus causes severe acute or late-onset encephalitis in human. In this study, we aimed to investigate the mechanisms of measles and nipah virus spread between neural cells. We established the AMCA (on-chip agarose microchamber array) system for primary neural cells, and elucidated the propagation manner of MV to neighboring cells. By using fluorescent labeled- viral protein, a new domain, which was important for interaction of NiV N and P protein, was identified. Further, we investigated NiV spread in African green monkey by using EGFP-expressing recombinant NiV. The results indicated that lethal infection to CNS occurred after systemic infection.