The exercise and dietary intervention study of the obesity gene

过氧化物酶体增殖物激活受体γ2(PPARγ2)基因与肥胖和代谢综合征有关。低水平的心肺适能也是METS发展的一个强有力的决定因素。这项横断面研究旨在探讨PPARγ2基因与心肺适合性之间的交互作用对蛋氨酸代谢综合征风险的影响。健康的日本男性(n=211)和女性(n=505)参与了这项研究。所有受试者按性别、体能水平(高、低体能组)、年龄(40岁以下)和中老年(40岁)分为8组。40年)。采用实时荧光定量聚合酶链式反应技术检测PPARTaq-Man基因γ-2基因(Pro12Ala和C1431T)。以年龄调整为协变量的双因素方差分析显示，适合性与PPARγ2基因C1431T CC基因型交互作用对青年男性METS风险有显著影响，且心肺功能适合度低的CC基因型组患METS的风险显著高于相应的CT+TT型或高适宜组。在任何组的中老年男性或女性中，在确定蛋氨酸代谢综合征风险方面，健康状况和基因型之间没有显著的交互作用。对于PPARγ2基因的Pro12Ala基因，适合度或基因效应没有显著差异，测量变量之间也没有任何交互作用。我们的结论是，PPARγ2基因中C1431T的CC基因型和低心肺适合度可能会增加年轻男性(40岁)患METS的风险，即使调整了年龄。

The peroxisome proliferator-activated receptor gamma 2(PPARγ2) genotypes are related to obesity and the metabolic syndrome(MetS). A low level of cardiorespiratory fitness is also a strong determining factor in the development of MetS. This cross-sectional study was performed to investigate the influence of the interaction between the PPARγ2 genotype and cardiorespiratory fitness on the risk of MetS. Healthy Japanese men(n=211) and women(n=505) participated in this study. All subjects were divided into 8 groups according to sex, fitness level(high and low fitness groups), and age(younger, age<40 years and middle-aged/older, age. 40 years). The PPARγ2 genotypes(Pro12Ala and C1431T) were analyzed by using real-time PCR with Taq-Man probes. Two-way ANCOVA with adjustment for age as a covariate indicated that fitness and the CC genotype of C1431T in the PPARγ2 gene interacted to produce a significant effect on MetS risk in younger men and that the risk of MetS in the CC genotype group with low cardiorespiratory fitness was significantly higher than that in the corresponding CT+TT genotypes or in the high fitness groups. There was no significant interaction between fitness and genotype in determining MetS risk in middle-aged/older men or in women in any group. With regard to the Pro12Ala genotype of the PPARγ2 gene, there were no significant differences in fitness or genotype effects nor were there any interactions between measurement variables. We concluded that the CC genotype of C1431T in the PPARγ2 gene together with low cardiorespiratory fitness may increase the risk of MetS in younger men(age<40 years), even with adjustment for age.