Development of high-resolution 2D-3D hybrid acoustic impedance microscope for biological use

我们提出了一种用于医疗和生物用途的新型超声显微镜。将靶材放置在塑料基板上，通过基板发射和接收超声波信号。在这一阶段的研究中，我们提高了空间分辨率，并观察了脑瘤模型和培养的胶质瘤细胞。对于脑肿瘤组织，我们通过量化“局部声阻抗粗糙度”成功地突出了肿瘤。在细胞大小观察方面，我们成功地观察到抗癌药物作用后胶质瘤细胞的形态变化。最后，通过横断面声阻抗图像的观察，成功地同时对肿瘤和培养细胞进行了三维观察。

We proposed a new ultrasonic microscope for medical and biological use. The target is placed on a plastic substrate, and ultrasonic signal is transmitted and received across the substrate. In this phase of research, we improved spatial resolution, and observed brain tumor model and cultured glioma cells. As for brain tumor tissue, we succeeded in highlighting the tumor by quantifying" local roughness of acoustic impedance". As for cell size observation, we succeeded in observing morphological change of glioma cells after being exposed to anticancer agent. Finally, we succeeded in three dimensional observation of tumor and cultured cells simultaneously with the observation of cross sectional acoustic impedance image.