Elucidation of molecular basis of insect resistance in mungbean using comparative genomics information

为了阐明野生绿豆（Vigna radiata）抗性的分子基础，我们利用大规模分离群体和3个绿豆BAC文库构建了抗性位点（Br）周围的精细连锁图谱和详细物理图谱。重组系及其后代表明，同一连锁群中的两个不同区域负责抗性。此外，RNA-Seq分析显示，在抗性绿豆株系的发育种子中，从每个含有抗性基因的区域中鉴定出了一个强表达的基因。这两个基因具有高度的同源性，但在其他植物物种中，这两个基因及其同源基因的功能尚未得到注解。

In order to elucidate the molecular basis of bruchid resistance derived from a wild accession of mungbean(Vigna radiata), we constructed a fine linkage map and a detailed physical map around the insect resistance locus(Br) using a large-scale segregating population and three mungbean BAC libraries. Recombinant lines and their progenies indicated that the two distinct regions in the same linkage group are responsible for the resistance. In addition, RNA-Seq analysis revealed one gene, which strongly expressed in developing seeds of the resistant mungbean lines, was identified from each region harboring the resistance gene(s). These two genes shared a high degree of homology, but the function of the genes as well as their homologous genes in other plant species has not been annotated yet.