Study on the molecular mechanisms of the fungal cell-wallβ-1, 6-glucan biosynthesis

β-1，6-葡聚糖是酵母细胞壁的重要组成部分，其生物合成的分子机制尚不清楚。Kre 6及其同源物Skn 1与糖苷水解酶具有序列同源性，是该过程所必需的。在本研究中，我们发现Kre 6和Skn 1与内质网驻留蛋白Keg 1相互作用，Kre 6/Skn 1的一部分必须定位在生长芽的质膜上以合成β-1，6-葡聚糖，它们的正确折叠和定位需要内质网中Keg 1和Cne 1的支持。

The molecular mechanism of biosynthesis ofβ-1, 6-glucan, an essential component of the yeast cell wall, is still unclear. Kre6 and its homologue Skn1, which have sequence homology with glycoside hydrolases, are required for this process. In this study, we discovered that Kre6 and Skn1 interact with the ER-resident proteins Keg1, a part of Kre6/Skn1 must localize to the plasma membrane of growing buds forβ-1, 6-glucan synthesis, and their proper folding and localization to the growing buds require support of Keg1 and Cne1 in the ER.