Function and distribution of of miRNA in pituitary adenoma

在本研究中，我们重点研究了通过miRNA调节垂体腺瘤细胞催乳素(PRL)的信号级联反应。对大鼠正常垂体(RPC)和大鼠垂体腺瘤细胞系GH3进行了比较分析，以确定miRNA呈负相关。随后，通过靶向siRNA来敲除每个miRNA，然后通过ELISA研究来评估PRL的分泌。并利用基因芯片技术对信号通路进行了分析。在GH3细胞中显著高于RPC的miRNA中，我们发现RNO-miR-101b、-191和-194与PRL的分泌有关。同时，转RNO-miR-101b基因的GH3细胞PRL及其受体表达降低。总之，GH3表达不同数量的miRNA，尤其是RNO-miR-101介导的PRL的产生。MiRNA有望成为治疗垂体腺瘤的新靶点。

In the present study, we focused on signaling cascade via miRNA regulating prolactin(PRL) in pituitary adenoma cell. Profiling analysis comparing rat normal pituitary(RPC) and GH3, rat pituitary adenoma cell line, was examined to identify miRNA showing negative co-relation. Subsequently, each miRNA was knocked-down by targeting siRNA followed by ELISA study to evaluate PRL secretion. Furthermore, signaling cascade was analyzed by cDNA microarray analysis. Among miRNA significantly elevated in GH3 cells than RPC, we found rno-miR-101b,-191, and-194 were related to PRL secretion. Meanwhile, GH3 cells transfected of rno-miR-101b gene revealed reduced expression of PRL and its receptor. In conclusion, GH3 expressed different amount of miRNA and in particular, rno-miR-101 mediated PRL production. The miRNA can be a promising target for the treatment of pituitary adenoma.