Development of cell microarray as devices for cell diagnosis, regulation of cell function, and recovery of target cells

通过表面引发聚合法将NiPAAm或MPC/N-琥珀酰亚胺基丙烯酸酯（N-succinimidylacrylate，MPC/N-succinimidylacrylate）聚合物点样于MA玻璃表面，制备了4种类型的微阵列（MA：孔尺寸=20、30、40、50μm ;间距=100μm）。在MA上的斑点周围涂覆聚（MPC）。以HL-60细胞或诱导的人多能干细胞（iPS）为典型细胞，与抗体反应后作为靶细胞的特异性结合位点，在MA上实现选择性细胞贴附。通过使用所开发的设备，还成功地对MA上的附着细胞进行了高通量诊断。通过降低细胞培养温度，90%以上的HeLa细胞贴附在PNiPAAm包被的MA点上，有效地脱落。从37℃到25℃。含有人类染色体（HAC）的微细胞（MC）与白色血细胞U937的融合率最高为0.1%，高于悬浮细胞融合率（0.001%）。该MAs作为细胞诊断、细胞功能调节和靶细胞回收的装置是有用的，并且具有实际用途。

Four types of microarrays(MA : well sizes=20, 30, 40, 50μm ; pitch=100μm) spotted with poly(N-isopropylacrylamide ; PNiPAAm) or biocompatible MPC polymers having anti-body as a specific binding for target cells were prepared by surface-initiated polymerization of NiPAAm or MPC/N-succinimidylacrylate on the glass surface of MA. Poly(MPC) was coated around spots on the MAs. Selective cell attachment was achieved by using HL60 cells or induced human pluripotent stem(iPS) cells as a typical cell on the MAs after being reacted with anti-body as a specific binding site for targeting cells. High-throughput diagnosis of attached cells on the MA was also succeeded by using developed equipments. Over 90% attached HeLa cells on the PNiPAAm coated spots of the MA were efficiently exfoliated by decreasing cell cultured temp. from 37℃to 25℃. Microcell(MC) s containing Human Artifical Chromosome(HAC) and U937 as white blood cell were higher fused on the MCs immobilized MA with a maximum of 0.1% than that of suspension cell fusion(0.001%). The MAs were of useful and for practical uses as devices for cell diagnosis, regulation of cell function, and recovery of target cells.