Dynamics of structural formation of osteoblastic cells responding to change in its peripheral mechanical environment

在本研究中，我们研究了感知外周机械环境变化的机制，以及机械因素对成骨细胞MC3T3-E1结构变化的影响，MC3T3-E1在骨的机械适应中起着重要作用。我们观察了成骨细胞在控制局灶黏附复合体下肌动蛋白应力纤维的重组过程。我们观察了固定细胞在基质上的肌动蛋白细胞骨架的形态特征，并证实了控制粘附诱导细胞和纤维的偏好取向。此外，我们发现微图案的底物增强了肌动蛋白应力纤维的形成。这些结果表明，肌动蛋白束的密度和肌动蛋白丝的内部张力以及细胞骨架中黏附复合物的分布是感知成骨细胞外周力学环境变化的重要因素之一。

In this study, we investigated the mechanism to sense changes in peripheral mechanical environment and effects of mechanical factors on structural changes of cytoskeleton in osteoblastic cells (MC3T3-E1) which plays an important role for mechanical adaptation of bone. We observed the reorganization process of actin stress fibers in osteoblastic cells under controlled focal adhesion complex. The morphological characteristics of the actin cytoskeleton on the substrate with/without micropatterning were observed for the fixed cells, and we confirmed that the controlled adhesion induced preferred orientation of the cells and fibers. Additionally, we found that the micropatterned substrate enhanced formation of actin stress fibers.These results suggest that density of actin-bundling and internal tensile forces in actin filaments and distribution of focal adhesion complex in cytoskeleton are one of the important factors to sense changes in peripheral mechanical environment in osteoblastic cell.