A NADH-dependent fiber-optic biosensor with a UV-LED excitation system

构建并测试了一种用于高选择性酒精蒸汽监测的光纤生物嗅探器。为了实现高选择性，传感器利用烟酰胺腺嘌呤二核苷酸（NADH）的荧光，这是由乙醇脱氢酶的酶促反应产生的。生物嗅探器是通过将湿酶固定化膜连接到光纤NADH测量系统来制造的。当乙醇蒸气暴露时，荧光强度显着增加，并具有与乙醇气体浓度相关的稳定值。

A fiber optic bio-sniffer for high-selective alcohol vapor monitoring with an UV-LED was constructed and tested. In order to achieve high selectivity, the sensor utilizes fluorescence of nicotineamide adenine dinucleotide (NADH), which is produced by the enzymatic reaction of alcohol dehydrogenase. The bio-sniffer was fabricated by attaching a wet enzyme immobilized membrane to the fiber-optic NADH measurement system. The fluorescent intensity significantly increased with a stable value related to the ethanol gas concentration when ethanol vapor was exposed.