Functional analysis of Kinesin superfamily protein in kidney development

在这项研究中，我们对Kif26b进行了功能分析，Kif26b是kinesin超级家族蛋白的成员。Kif26b在后肾间充质中表达，后肾间充质是一种多能肾祖细胞。Kif26b的破坏导致肾脏发育不全，因为输尿管芽吸引受损。在kif26b缺失的后肾中，输尿管芽附近的间充质细胞之间的紧密粘附和整合素α 8的极化分布被破坏，导致输尿管芽引诱剂Gdnf的维持失败。体外过表达Kif26b通过与非肌球蛋白相互作用引起细胞粘附增加。因此，Kif26b对肾脏发育至关重要，因为它调节与输尿管芽接触的间充质细胞的粘附。

In this study, we performed the functional analysis of Kif26b, a member of kinesin super family proteins. Kif26b is expressed in the metanephric mesenchymes, a population of multipotent kidney progenitors. Disruption of Kif26b causes kidney agenesis because of impaired ureteric bud attraction. In the Kif26b-null metanephros, compact adhesion between mesenchymal cells adjacent to the ureteric buds and the polarized distribution of integrin alpha 8 were impaired, resulting in failed maintenance of Gdnf, a critical ureteric bud attractant. Overexpression of Kif26b in vitro caused increased cell adhesion through interactions with nonmuscle myosin. Thus, Kif26b is essential for kidney development because it regulates the adhesion of mesenchymal cells in contact with ureteric buds.