Organohalide respiration in the subseafloor microbial ecosystem-Investigation of RDH genes and dehalogenation activity

海洋沉积物中的卤化有机物是厌氧微生物呼吸的电子受体来源之一。在秘鲁东南太平洋、赤道东太平洋、俄勒冈州胡安·德·富卡海岭侧面和日本西北太平洋的沉积物中，共检测到32个推定的还原脱卤酶同源基因，这些沉积物采集的最大深度为海底以下358.6 m。此外，还观察了南开海槽前弧盆地沉积物浆体对2,4,6-三溴酚、2,4,6-三碘酚和三氯乙烯的脱卤活性。这些结果表明，在海底微生物生态系统中，脱盐呼吸是一个重要的产能途径。

Halogenated organic matter incorporated in marine sediments is one source of electron acceptors for anaerobic microbial respiration. A total of 32 putative reductive dehalogenase homologous genes were detected in sediments from the southeast Pacific off Peru, the eastern equatorial Pacific, the Juan de Fuca Ridge flank off Oregon, and the northwest Pacific off Japan, collected at a maximum depth of 358.6 m below the seafloor. In addition, dehalogenation activity toward 2,4,6-tribromophenol, 2,4,6-triiodophenol, and trichloroethene was observed in sediment slurry from the Nankai Trough Forearc Basin. These results suggest that dehalorespiration is an important energy-yielding pathway in the subseafloor microbial ecosystem.