Linkage mapping and eQTL analysis of candidate resistant gene for pine wilt disease caused by wood nematode

利用已报道的松材线虫抗性候选基因的EST信息进行3‘-RACE，以确定EST的3’端序列。设计聚合酶链式反应引物，对大配子体植物单倍体基因组进行测序，筛选出单基因。为了扩增含有SNPs的单基因，开发了两个引物。对病原菌相关基因的表达进行QTL分析。几丁质酶和PR-4的EQTL在同一抗性表型性状连锁群中检测到，提示这两个基因与抗性有相似的调控因子

3'-RACE was conducted using previously reported EST information of candidate resistant gene for pine wood nematode in order to determine 3'-end sequence of the ESTs. PCR primers were designed and single-genes were screened by sequencing haploid genome extracted from megagametophyto. Two primers were developed to amplify single-genes containing SNPs. Expression QTL analysis of pathogen related genes were conducted. EQTL of chitinase and PR-4 were detected in the same linkage group of phenotypic resistant trait, suggesting similar regulation factor was related to these genes and resistance