Functional analysis of amplification and overexpression of c-Met in lung adenocarcinoma

I组(n=22)：(1)高表达TTF1、MUC1CK7；(2)高磷酸化EGFR、MET；(3)频繁突变或扩增EGFR、MET、HER2；(4)高表达E-钙粘素、HER3、MET、u-PA、COX-2、层粘连蛋白γ2；(5)对吉非替尼敏感、对顺铂耐药；II组(n=18)：(1)TTF-1、MUC1和CK7三重阴性；(2)EGFR和MET无或少量磷酸化；(3)EGFR、MET和HER2无突变或扩增；(4)FGFR1、Vimentin和TCF8高表达；(5)对吉非替尼耐药和对顺铂敏感(Matsubara等，Am J Pathol，2010)。

We have demonstrated that the 40 cell lines could be classified into two groups: Group I (n=22) characterized by (1) high expressions of TTF-1, MUC1,and CK7, (2) high phosphorylation of EGFR and MET, (3) frequent mutation or amplification of EGFR, MET and HER2, (4) high expressions of E-cadherin, HER3, MET, u-PA, Cox-2, and laminin γ2 and (5) sensitivity to gefitinib and resistance to cisplatin; Group II (n=18) characterized by (1) triple-negative for TTF-1, MUC1 and CK7, (2) no or a little phosphorylation of EGFR and MET, (3) no mutation or amplification of EGFR, MET and HER2, (4) high expressions of FGFR1, vimentin and TCF8 and (5) resistance to gefitinib and sensitivity to cisplatin(Matsubara et al, Am J Pathol, 2010).