Development of novel alternative approach for liver cirrhosis therapy using adipose-derived stem cells

本研究旨在探讨脂肪干细胞(ADSC)骨骼肌内移植治疗小鼠肝硬变的疗效。将人ADSC移植到裸小鼠的腓肠肌内，反复注射CCl4诱导肝损伤和肝纤维化。移植ADSC的小鼠血清白蛋白和总蛋白水平维持在高于对照组的水平。ADSC组小鼠的脾指数(组织重量/体重)显著低于对照组，其他骨骼肌重/体重也保持不变。作为纤维化指标的α-平滑肌肌动蛋白在ADSC小鼠中的蛋白表达也低于对照组。本研究提示，ADSC骨骼肌内移植有望成为肝硬变患者替代治疗的新策略之一。

The purpose in the present study was to investigate the effectiveness of adipose-derived stem cell(ADSC) transplantation into skeletal muscle as novel alternative therapy for liver cirrhosis in mouse. Human ADSC was transplanted into the gastrocnemius muscle in nude mouse, and then, the mouse received the repetitional injections of CCl_4 to induce liver damage and fibrosis. In the ADSC-transplanted mice, serum levels of albumin and total protein were kept at higher than in the control mice. Spleen index(tissue weight/body weight) were significantly lower in the ADSC mice than the control, and other skeletal muscle weights per body weight were also maintained in the ADSC mice. The protein expression of alpha-smooth muscle actin as a parameter of fibrosis was also lower in the ADSC mice than the control. The present study suggested a possibility that the transplantation of ADSC into skeletal muscle would be one of novel strategies for alternative therapy to the patients with liver cirrhosis.