Establishment of tracking method for induced pluripotent stem cells using 64-Cu positron emitting isotope

铜正电子发射同位素标记诱导多能干细胞(IPSCs)是利用PTSM材料作为结合材料的一项技术成功。并成功获得了IPSC分布的正电子发射断层扫描(PET)图像。采用两种给药途径：1)尾静脉给药，2)左心室直接给药。各分布间差异有统计学意义。静脉注射仅见肺部分布。另一方面，描述了其在脑、肾和移植瘤中的分布。由于-铜的半衰期为13小时，因此在注射标记细胞后24小时即可获得正电子发射计算机断层扫描图像。与18F-FDG标记的IPSCs图像相比，注射正电子标记细胞后6h内，-铜标记图像的信噪比低于18F-FDG标记的图像。

Induced pluripotent stem cells (iPSCs) Labeling by 64-Cu positron emitting isotopes is a technical success with using PTSM material as a binding material. And positron emission tomography (PET) images which indicate iPSC distributions were acquired successfully. Two administration pathways were used ; 1) intravenous injection via tail vein, 2) left ventricle injection directly. There was significant difference between each distribution. Only lung distribution was seen by intravenous injection. On the other hand, the distributions to brain, kidneys, and transplanted tumors were described. Since half-life for 64-Cu is 13 hours, PET images could be acquired at 24 hours after labeled cells injection. Comparison with images for 18F-FDG labeled iPSCs, 64-Cu labeled image has less signal-to-noise ratio than that of 18F-FDG within 6 hour after positron-labeled cells administration.