Development of new gene knockdown techniques for platelets using miRNA

将miR RNAi表达载体转染人CD 3 - 4+祖细胞和小鼠骨髓细胞，体外培养3- 4周，诱导分化为血小板，证实目的基因在血小板细胞中被敲除。这些血小板对激动剂的反应具有较低的聚集能力和P-选择素表达。在小鼠实验模型中，通过注射这些基因敲低的血小板，存活率和肺栓塞的严重程度得到改善。有必要进行进一步研究，以进行详细分析。

After transfection of miR RNAi expression vector in human CD34+ progenitor cells and mouse bone marrow cells along with differentiating into platelets with 3-4week culture in vitro, we have confirmed the target gene knocked down in platelet cells. These platelets have less ability of aggregation and P-selectin expression in response to agonists. With injection of these gene knocked down platelets, survival and severity of pulmonary embolism have improved in mouse experimental model. Further studies are necessary to undergo detailed analyses.