Investigation of differentiation induction method of capillary endothelial cells and pericytes by using adipose derived stem cells

基于对损伤脂肪组织渗出液的分析，我们制备了一种含有与渗出液相同比例的损伤相关生长因子的混合物，称为脂肪损伤鸡尾酒（AIC）。本研究的目的是阐明AIC的治疗潜力。与血管内皮细胞相比，AIC优先激活脂肪干/祖/基质细胞（ASCs）增殖、迁移和形成网络。AIC处理的ASCs倾向于分化成脂肪细胞或血管构成细胞，而不是分化成其他细胞类型。在由手术干预或糖尿病诱导的小鼠缺血性脂肪组织中，AIC施用增强增殖，特别是CD 31-/CD 34 + ASC的增殖，并通过增加毛细血管密度和减少纤维形成来减轻组织缺氧。这些结果表明，AIC可能通过激活ASC和其他细胞诱导脂肪重塑和新血管形成，对各种缺血/缺氧状况具有治疗潜力。与基于细胞的疗法相比，AIC治疗在发病率、成本和物理风险方面具有许多优势，并且可以用作改善组织氧的替代疗法。

Based on the analysis of exudates from injured adipose tissue, we prepared a mixture containing the injury-associated growth factors at the same proportion as the exudates, named adipose injury cocktail (AIC). The purpose of this study is to elucidate the therapeutic potentials of AIC. AIC preferentially activated adipose-derived stem/progenitor/ stromal cells (ASCs) to proliferate, migrate, and form networks compared with vascular endothelialcells. AIC-treated ASCs tended to differentiate into adipocytes or vessel-constituting cells rather than into other cell types. In ischemic adipose tissues of mice, induced by either a surgical intervention or diabetes, AIC administration enhanced proliferation, especially of CD31-/CD34+ ASCs, and mitigated tissue hypoxia by increasing capillary density and reducing fibrogenesis. These results suggest that AIC may have therapeutic potentials for various ischemic/hypoxic conditions by inducing adipose remodeling and neovascularization through activation of ASCs and other cells. Treatment with AIC has many advantages over cell-based therapies regarding morbidity, cost, and physical risks and may be used as an alternative therapy for improving tissue oxygen.