Cryopreservation and dried preservation at room temperature of human skin by vitrification procedure.
Cryopreservation and dried preservation at room temperature of human skin by vitrification procedure.
批准号:
21791745
负责人:
TOKUYAMA Eijiro
金额:
$2.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
生物组织的低温保存需要特殊的设备。玻璃化法可以解决这一问题。已知玻璃化程序增加冷冻保存的细胞存活率。采用玻璃化冷冻法对人和大鼠皮肤进行了冷冻保存和室温干燥保存。此外,我们还进行了鼠皮移植。透射电子显微镜观察细胞结构保持不变,但细胞不能存活。
英文摘要
Cryopreservation of biological tissue needs special facilities. Vitrification procedure may solve this problem. It is known that vitrification procedure increases cryopreserved cell survival rate. We carried out cryopreservation and dried preservation at room temperature of human and rat skin by vitrification procedure. Besides, we carried out rat skin grafting. We could confirm cell structure maintain by transmission electron microscopy, but cells could not survive.
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会议论文
Mechanical stretch on Human skin equivalents with a new peptide scaffold
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批准号:24592713
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.41万
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财政年份:2012
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负责人:TOKUYAMA Eijiro
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依托单位: