Effects of ATP receptor on sex difference of chronic muscle pain(temporomandibular disorder)

由于性激素被认为在这些疾病中起作用，我们的研究检查了卵巢切除术与雌激素替代或不雌激素替代对大鼠肌肉疼痛发展的影响。将sd - dawley大鼠平均分为雄性组（male）、去卵巢雌性组（OVX）和去卵巢补雌激素雌性组（OVX+E_2）。所有大鼠左肠肌注射3%λ-卡拉胶（0.1 ml, im）。我们随后测量了注射前OVX组和男性组的疼痛阈值高于OVX+E_2组。注射后1 ~ 2天，各组疼痛阈值急剧下降，均为最低。除OVX+E_2组外，其余组在第6周恢复到基线水平。另一方面，术后将P2XR拮抗剂PPADS（100μg）或磷酸盐缓冲盐水（作为对照）持续灌注于MDH上。麻醉大鼠的伤害特异性（NS），在神经元特性基线值后进行评估。在男性和OVX组中，PPADS产生了反映CS的长时间和神经可塑性变化，即神经元感受野大小和挤压诱发反应增加，激活阈值降低。相比之下，在OVX+E_2组中，PPADS也产生了神经元性质的变化。这些数据表明P2X受体可能不参与MDH伤害感觉神经元CS的启动。通过功能形态学研究，探讨针刺对膀胱过度活动模型大鼠欲空传递系统的作用机制。乙酸致膀胱刺激大鼠和氯致膀胱炎大鼠经骶针刺激后，间缩间期增加。观察骶部针刺对膀胱粘膜恢复过程的影响。骶针刺激可通过不同于药物的作用机制有效改善尿频，并可预期对耐受性过动膀胱的作用。少

Since sex hormones are believed to play a role in these ailments, our study examined the influence of ovariectomy with or without estrogen replacement on the development of muscle pain in rats. Sprague-Dawley rats were equally divided into a male group(Male), ovariectomized female group(OVX), and ovariectomized, estrogen-supplemented female group(OVX+E_2). All rats were injected with 3%λ-carrageenan(0.1 ml, im) in the left gastrocnemial(GS) muscle. We subsequently measured pain thresholds Pain thresholds in OVX and Male groups were higher than in the OVX+E_2 group prior to injection. Pain thresholds decreased sharply and were at their lowest in all groups 1-2 days post injection. They returned to baseline levels by week 6 in all but the OVX+E_2 group.On the other hand, after surgery, the P2XR antagonist PPADS(100μg) or phosphate-buffered saline(as control) was continuously superfused over the MDH. Nociceptive-specific(NS) in anesthetized rats, after baseline values of neuronal properti … More es were assessed. In the Male and OVX group, PPADS produced prolonged and neuroplastic changes reflecting CS, i. e., increased neuronal receptive field size and pinch evoked responses and decreased activation threshold. In contrast, in the OVX+E_2 group, PPADS also produce to changes in neuronal properties. These data suggest that P2X receptors may be not involved in the initiation of CS in MDH nociceptive neurons.This study of functional morphology was performed to investigate the action mechanism of the acupuncture for the transfer system of desire-to-void using overactive bladder model rats. The intercontraction interval was increased immediately after sacral acupuncture stimulation in acetic acid-induced bladder irritation rats and chloride-induced cystitis rats. And we observed the change of the recovery course of bladder mucosa by performing a sacral acupuncture. Sacral acupuncture stimulus is effective in improving urinary frequency by different action mechanism from medication, and can expect the effect on the overactive bladder of tolerance. Less