Toward the development of biomimetic under-water adhesives inspired by barnacle larval cement

为了开发以藤壶幼虫粘合剂为灵感的仿生水下粘合剂，我们获得了以下结果：1)我们发展了一种利用原子力显微镜测量海洋粘合剂宏观作用力的方法。2)我们开发了一种使用罗氏玻璃毛细管在模拟海洋环境中测量蛋白质和粘土纳米片之间相互作用的微量分析方法。3)我们制备了两种重组水泥蛋白：rec-CCG-57k和rec-CCG-ASP，但这两种重组蛋白对1)和2)均无积极作用。4)我们发现rec-CCG-57K对海洋革兰氏阴性菌--柑橘扁平球菌有很强的抗菌活性。5)在利用下一代测序技术寻找未知水泥组分的过程中，我们发现了一个与玫瑰花成体水泥蛋白基因MRCP-100k同源的基因。

Toward the development of biomimetic under-water adhesives inspired by larval cement of the barnacle, Megabalanus rosa, we have obtained the following results.1) We have developed a method to measure macroscopic forces for marine adhesives using an atomic force microscope. 2) We have developed a microassay to measure the interaction between proteins and clay nanosheets in mock-marine environments using Roche glass capillaries. 3) We have prepared two recombinant cement proteins ; rec-Ccg-57k and rec-Ccg-ASP, both of which, however, did not show positive effects on 1) and 2). 4) We found the strong antimicrobial activity of rec-Ccg-57k against marine GRAM-negative bacteria, Planococcus citreus. 5) In the course of searching unidentified cement components using next generation sequencing, we have found a gene homologous to M. rosa adult cement protein gene mrcp-100k.