Development of a novel estimation method of cell functions by creating non-adhesive single cell culture technology

以聚乙二醇和精氨酸-甘氨酸-天冬氨酸为主要原料，制备了聚乙二醇- grgds。原代肝细胞在PEG- grgds治疗下分散取决于PEG的大小。经PEG-GRGDS处理的肝细胞存活率提高，细胞骨架组织改善，白蛋白生成和药物代谢活性表达良好。因此，利用PEG-GRGDS建立非黏附单细胞培养技术，成功开发了一种新的细胞功能估计方法。

PEG-GRGDS consisting of polyethylene glycol and arginine-glycine-aspartic acid was prepared. Primary hepatocytes were dispersed by PEG-GRGDS treatment depending on a PEG size. The hepatocytes treated with PEG-GRGDS improved their viability with the cytoskeleton organization, and well expressed albumin production and drug metabolism activities. Therefore, a novel estimation method of cell functions by creating non-adhesive single cell culture technology using PEG-GRGDS was successfully developed.