Exploring for new bioactive peptides using our novel methods

未知生物活性肽的发现有助于阐明循环系统的复杂性。在过去的20年里，我们已经发现了几种有效的降压和血管加压肽。G .肾上腺髓质素，其相关肽和原血管紧张素-12)。此外，我们还阐明了基于ramp1的G蛋白偶联受体（gpcr）的信号传导、转运和病理生理作用的机制。在这项研究中，我们试图鉴定新的生物活性肽，这些肽可能能够与循环系统中的人类孤儿gpcr结合。我们使用了三种针对激动剂诱导的构象变化、磷酸化和内化的新检测方法。此外，我们还发现了一种鉴定新肽的新策略。然而，我们无法确定任何生物活性肽的预期目的。1 .缩写RAMP代表受体活性修饰蛋白。

The discovery of unknown bioactive peptides can help to clarify the complexity of the circulatory system. In the past 20 years, we have discovered several potent hypotensive and vasopressor peptides(e. g., adrenomedullin, its related peptides and proangiotensin-12). Furthermore, we have clarified the mechanisms of the signaling, trafficking and pathophysiological roles of the RAMP1-based G protein-coupled receptors(GPCRs). In this study, we have attempted to identify novel bioactive peptides that may be able to bind to the human orphan GPCRs in the circulatory system. We used our three novel assays targeting the agonist-induced conformational change, phosphorylation and internalization of many GPCRs. Furthermore, we found a new strategy for the identification of novel peptides during this research. Nevertheless, we were unable to identify any bioactive peptides for the intended purpose. 1The acronym RAMP represents receptor activity-modifying protein.