Studies on resin allergy using murine models

在项目开始时，我们认为我们已经通过实验成功地建立了树脂过敏的小鼠模型，使用的是临床上用于牙科的树脂单体甲基丙烯酸甲酯(MMA)的制剂。然而，在后来的实验中，使用试剂等级的MMA，我们无法复制它，这导致我们推测“LM可能促进了对LM以外的过敏原的过敏”。金属过敏是与牙科相关的过敏，发病率很高。因此，我们研究了黄连对镍过敏的佐剂作用。我们发现甲基丙烯酸甲酯和甲基丙烯酸羟乙酯(HEMA)都是炎症性的，并促进小鼠的镍过敏。各种微生物物质和含氮双膦酸盐(NBPS，抗骨吸收药物)也会促进镍过敏。这些发现表明，炎性化学物质以及口腔细菌可能会促进金属过敏，作为免疫佐剂。

At the start of the project, we were thinking that we had succeed in establishing the murine model of resin allergy by experiments using a preparation of resin monomer methyl methacrylate(MMA)that is clinically used in dentistry. However, in later experiments using a reagent grade of MMA, we could not reproduce it, leading us to speculate that"LM might promote allergies to allergens other than LM". Metal allergies are dentistry-associated allergies with a high incidence. Thus, we examined the adjuvant effect of RM on Ni-allergy. We found that both MMA and 2-hydroxyethyl methacrylate(HEMA)are inflammatory and promote Ni allergy in mice. Various microbial substances and nitrogen-containing bisphosphonates(NBPs, anti-bone-resorptive drugs)also promoted Ni allergy. These findings suggest that inflammatory chemicals, as well as oral bacteria, may promote metal allergies as an immunological adjuvant.