The analysis of relations of the chemokines which human gliomas produce and the tumor infiltrating macrophage/Microglia

单核细胞趋化蛋白3（MCP-3）属于CC趋化因子的MCP亚群，并且促进免疫细胞的趋化性。MCP-1被认为在单核细胞浸润到肿瘤组织中起重要作用;然而，肿瘤浸润性巨噬细胞/小胶质细胞（TIM/M）和趋化因子表达之间的关系尚未在人类胶质瘤样品中详细研究;因此，我们首先使用实时PCR检测了人类肿瘤细胞系（包括胶质瘤系）中几种趋化因子和趋化因子受体的表达。我们发现几种胶质瘤细胞系主要表达MCP-3，而不是MCP-1。为了评估MCP-3在人脑胶质瘤组织中表达的意义，我们随后检测了CD 68 + TIM/M的数量、TIM/M在总细胞群中的百分比以及MCP-1和MCP-3在人脑胶质瘤组织中的表达。TIM/M的百分比与MCP-3表达水平之间存在相关性;然而，TIM/M的百分比与MCP-1表达之间没有相关性。TIM/M数目与患者预后无相关性。这些数据表明，肿瘤细胞来源的MCP-3，而不是MCP-1，促进巨噬细胞/小胶质细胞浸润到肿瘤组织。这是首次明确比较MCP-3与MCP-1在TIM/M肿瘤浸润率中的意义的研究。

The monocyte chemotactic protein 3 (MCP-3) belongs to the MCP subgroup of the CC chemokines and promotes chemotaxis of immune cells. MCP-1 is believed to play an important role in monocyte infiltration into tumor tissues ; however, the relationship between tumor-infiltrating macrophage/microglia (TIM/M) and the expression of chemo- kines has not been investigated in detail in human glioma samples ; therefore, we first examined the expression of several chemokines and chemokine receptors in human tumor cell lines, which included glioma lines, using real-time PCR. We found that several glioma lines expressed MCP-3 predominantly, and not MCP-1. In order to assess the significance of MCP-3 expression in human glioma tissues, we then examined the number of CD68+ TIM/M, the percentage of TIM/M in the total cell population, and the expression of MCP-1 and MCP-3 in glioma tissues. There was a correlation between the percentage of TIM/M and MCP-3 expression levels ; however, there was no correlation between the percentage of TIM/M and MCP-1 expression. There was no correlation between the number of TIM/M and prognosis of patients. These data indicate that tumor cell-derived MCP-3, but not MCP-1, facilitates the infiltration of macrophage/ microglia into tumor tissues. This is the first study that clearly compared the significance of MCP-3 with that of MCP-1 in the tumor infiltration rates of TIM/M.