Analysis of molecular mechanism of neuron-glia interaction in long-term memory formation

最近的研究表明，神经胶质细胞通过神经元-神经胶质细胞的相互作用参与突触的形成和可塑性。然而，它们对持久记忆的贡献尚不清楚。以前我们发现，长时记忆(LTM)的形成需要依赖训练的Drodexila Klingon(KLG)的增加，KLG是一种嗜同性细胞黏附分子。在本研究中，我们通过对KLG的功能分析来阐明神经元-胶质细胞相互作用在LTM形成中的作用。KLG蛋白在神经元和神经胶质细胞中都有表达，需要KLG介导的训练依赖的神经元-胶质细胞相互作用的增加才能形成LTM。此外，我们发现神经元-神经胶质细胞之间的相互作用通过KLG调节LTM形成所需的胶质基因表达。

Recent studies suggest that glias are implicated in synapse formation and plasticity through neuron-glia interaction. However, how they contribute to the long-lasting memory is not clear. Previously we found that training-dependent increase in Drosophila Klingon (Klg), a homophilic cell adhesion molecule, is required for long-term memory (LTM) formation. In this study we purposed to elucidate the function of neuron-glia interaction in LTM formation through the functional analysis of Klg. Klg protein was expressed in both neurons and glia, and a training-dependent increase in neuron-glia interactions mediated by Klg was required to form LTM. Furthermore, we identified that neuron-glia interactions via Klg modulate glial-gene expression required for LTM formation.