Study on enhancement of transdermal vaccination by photothermaleffect

角质层是蛋白质透皮给药的主要屏障。我们尝试利用金纳米棒的光热效应来增强角质层的通透性。将卵清蛋白与金纳米棒混合，然后应用于皮肤。近红外光照射后，卵清蛋白在皮肤内移位，并产生抗卵清蛋白抗体。当使用脉冲激光时，检测到蛋白质移位而不加热皮肤的更深部分。这些数据对皮肤接种系统的开发具有重要意义。

Stratum corneum is major barrier of transdermal protein delivery. We tried enhancingpermeability of stratum corneum by photothermal effect of gold nanorods. Ovalbumin wasmixed with gold nanorods, then, applied on skin. After irradiation of near infrared light,translocation of ovalbumin into skin and production of anti-ovalbumin antibody wereobserved. When a pulsed-laser was used, protein translocation without heating deeper partof skin was detected. These data would be important for development of dermal vaccinationsystem.